International Journal of Fundamental and Applied Sciences (IJFAS)

Exploring the Diversification in Grafted Copolymer

Ashish Chauhan — 2012-06-30

The paper deals with optimization of the reaction parameters, graft copolymerization, characterization
and evaluation of the transformations in Roselle stem fiber on graft copolymerization with vinyl monomer, using ceric
ammonium nitrate nitric acid initiator system. Methods: Different reaction parameters such as temperature, time, initiator
concentration, monomer concentration and pH were optimized to get the maximum graft yield. The graft copolymer thus
formed were characterized by advanced techniques. Results: The physico-chemico-thermal resistance, moisture absorbance,
swelling behavior of graft copolymers and the dye uptake behavior were studied and found to have improved. Conclusion:
Hence, this first report of novel graft copolymers is to help towards various applications.

Determination of genetic fidelity of Micropropagated plants of Zingiber officinale cv-Majhauley of Sikkim Himalaya using RAPD markers

Sushen Pradhan — 2012-06-30

bstract
The present investigation was carried out for the determination of genetic conformity of micropropagated plants of Zingiber
officinale cv Majhauley using Random amplified polymorphic DNA (RAPD) technique.Total fifteen different arbitrary
decamers were used as primers to amplify DNA from in vitro plant material to assess the genetic fidelity of 18 months old in
vitro cultured plantlets. Total 38 amplified reproducible bands were produced from 10 primers with an average of 3.8 per
RAPD primer, amplified bands were ranging from 150-950 base pair, the highest seven number of bands found in primer
CLT53 (50-875 bp) and lowest one band in CLT 112 (575 bp) and CLT 192 (650 bp) primers. All the primers were found to be
monomorphic and no genetic variation was detected within the micropropagated plants. Present protocol may be used for
testing of ginger propagules to ensure mass production of disease free planting materials

An in vitro new vista to identify hypoglycemic activity

Sushil Kumar Middha — 2012-06-30

The present study aimed to detect a novel in-vitro method for some of indigenous plant
extracts which demonstrate inhibitory effect to glucose oxidase and are in use of our traditional remedial system for
hypoglycemic impending.
Result: Bambusa vulgaris possessed hypoglycemic activity of varying degree. Dendrocalamus hamiltonii,
Dendrocalamus sikkimensis had shown the better activity in neutral and basic media than others. Whereas, Bambusa
balcooa and Bambusa pallida showed prominent result in acidic media. The result in three different media revealed
that, acidic medium had less prominent hypoglycemic activity as compared to neutral and basic medium.
Conclusion: This may be the first report for using glucose oxidase as in vitro method to prove their antidiabetic
properties, can save a hugh preclinical burden for the budding herbal products